ABSTRACT
OBJECTIVE@#To investigate changes of LDH and HBDH activity in rabbit serum after non-thermal low voltage electrical injury and to provide diagnostic criteria for non-thermal low voltage electrical injury.@*METHODS@#Forty New Zealand rabbits were randomly distributed into control group and electrical injury group (EI-groups; designated 7 time points: 0 h, 2 h, 4 h, 12 h, 1 d, 2 d, 3 d), 5 rabbits per each group. EI-groups were treated with the method of non-thermal low voltage electrical injury established in our laboratory. Ventricular blood (5 mL) was obtained under anesthesia at designated time points after electrical injury. The activities of LDH and HBDH were measured.@*RESULTS@#Dynamic changes were observed with certain patterns from target serum enzyme activities after electrical injury. Compared with control group, the activities of LDH increased markedly at 4 h, 12 h, and on days 1, 2, and 3 after injury (4 h, 12 h, and day 1 P<0.01; day 2 and day 3 P<0.05). Activities of HBDH increased markedly at day 2 and day 3 after injury (P<0.05). The ratio of HBDH/LDH decreased markedly at 2 h, 4 h, and 12 h after injury (P<0.01).@*CONCLUSION@#Dynamic changes of LDH and HBDH activities may be useful in diagnosis of non-thermal low voltage electrical injury and in estimation of post injury intervals.
Subject(s)
Animals , Female , Male , Rabbits , Electric Injuries/enzymology , Forensic Pathology , Hydroxybutyrate Dehydrogenase/blood , L-Lactate Dehydrogenase/blood , Random AllocationABSTRACT
Serum alpha-hydroxybutyrate dehydrogenase (HBDH) and lactate dehydrogenase isoenzyme (LDH-1) levels by two techniques viz., adsorption on DEAE Sephadex A-50 and by electrophoresis were estimated in healthy subjects and patients with testicular tumours. The correlation coefficient between HBDH and LDH-1 by adsorption technique was found to be r = 0.8. HBDH was found to have a value almost twice that of LDH-1 by adsorption and electrophoretic technique and the activity of LDH-1 obtained by two methods and of HBDH in normals and patients with testicular tumours could be converted using a factor of 0.557. The quantitative method used for determination of HBDH is reliable, accurate, simple and rapid and therefore has better value in a clinical setting than electrophoresis and adsorption techniques which are laborious and time consuming.
Subject(s)
Chromatography, Ion Exchange , Electrophoresis , Humans , Hydroxybutyrate Dehydrogenase/blood , Isoenzymes , L-Lactate Dehydrogenase/blood , Male , Testicular Neoplasms/bloodABSTRACT
Serum levels of Lactate Dehydrogenase (LDH), alpha-Hydroxybutyrate Dehydrogenase (HBDH) and the relative ratio of alpha-Hydroxybutyrate Dehydrogenase to Lactate Dehydrogenase (HBDH/LDH) were determined in 51 cases of histologically proved testicular germ cell tumors and 40 healthy controls. Serum LDH and HBDH levels were found to be raised in both seminomatous and non-seminomatous germ cell tumors. The ratio of HBDH/LDH was found to be statistically significant in seminomas and insignificant in non-seminomatous germ cell tumors. Furthermore, the levels of LDH and HBDH showed a fall, following treatment indicating response to therapy. Serum LDH and HBDH could both be used as tumor markers in the diagnosis of testicular germ cell tumors as well as prognostic indicators in monitoring therapy, HBDH, being more specific in monitoring therapy as compared to serum LDH.
Subject(s)
Adolescent , Adult , Humans , Hydroxybutyrate Dehydrogenase/blood , L-Lactate Dehydrogenase/blood , Male , Middle Aged , Neoplasms, Germ Cell and Embryonal/enzymology , Testicular Neoplasms/enzymologyABSTRACT
Foram estudados prospectivamente, com distribuiçäo aleatória, 27 pacientes submetidos a implante de prótese valvular aórtica com circulaçäo extracorpórea, alterando-se o método de proteçäo miocárdica entre a hipotermia local isolada (HL), cardioplegia potássica em soluçäo cristalóide (CC) e cardioplegia potássica em sangue oxigenado (CS). Foram realizadas dosagens das enzimas CPK, DHL, HBDH e TGO da 2ª à 96ª hora de pós-operatório. O grupo submetido à HL (6 casos) apresentou níveis enzímicos no pós-operatório discretamente mais altos em relaçäo aos grupos CC (10 casos) e CS (11 casos). A comparaçäo entre estes 2 grupos evidenciou uma queda mais rápida dos valores enzímicos no pós-operatório do grupo CS. O número de pacientes em classe funcional III-IV (NYHA) näo foi significativamente diferente nos três grupos. Nenhum paciente apresentou níveis séricos de CPK >= 1100 e de TGO >= 80 UI/1. Um paciente do grupo CS (10,0%) e outro no CC (9,1%) necessitaram de suporte farmacológico no pós-operatório, näo sendo as diferenças entre os três grupos significativas. Concluiu-se que a proteçäo miocárdica provida por qualquer dos três métodos foi clinicamente satisfatória, permitindo a análise enzímica distinguir diferença significativa entre os meses em alguns intervalos pós-operatórios